Aim and Objective: Chromenes are one of the most demanded compounds that are recognized for
their unique pharmacological and biological activities. This report illustrates the synthesis of new chromene
series and chromene based azo chromophores as first example and examines their biological applications in
order to explore their possible biological and cytotoxic activities.
Material and Method: Using multicomponent methodology, two new families of 4H-chromene and chromene
azo chromophores have been successfully synthesized. These compounds were evaluated for their antimicrobial
activity as well as their cytotoxicity against three cancer cell lines. The docking simulations were performed
for their structure-activity relationship analyses.
Results: Novel chromene and chromene azo chromophores have been synthesized successfully using multicomponent
methodology. Several of these compounds showed significant potent antimicrobial activities
compared to reference drugs. In addition, antiproliferative analysis against three target cell lines was examined.
Compounds 4a, 4b, 4c, and 7c possessed significant antiproliferative activity with an IC50 of 0.3 to 2 µg/mL,
while their docking study proved that the pharmacophore features played a crucial role in reducing the regulation
of cancer cell signaling or apoptotic activation.
Conclusion: New derivatives of benzo chromene were obtained via multicomponent methodology. The new
compounds were examined for their antimicrobial and antiproliferative activities. Molecular docking study was
carried out to clarify the structural desires and target binding properties as a trial for explaining the structureactivity