Background: Recent studies have sought to draw attention of biological activity
of Campylandra aurantiaca. The aim of the present study was to evaluate the effect
of flavonoid enriched fraction of Campylandra aurantiaca (FEFCA) on in vitro and
in vivo antioxidant and DNA protective effect in mouse peritoneal macrophages cells.
Methods: FEFCAwas characterized by HPLC analysis. The in vitro antioxidant activities
of FEFCA was measured by different in vitro assays like 1, 1-diphenyl-2-picrylhydrazil
radical (DPPH), superoxide anions, nitric oxide and hydroxyl radicals scavenging methods.
Isolated mouse peritoneal macrophages were oxidized by carbon tetra chloride (CCl4)
in animal model; subsequently the protective effect of FEFCA was determined in terms of
estimation of antioxidant enzyme and the damage to DNA of the cells.
Results: FEFCA exhibited both in vitro antioxidant activities in a concentration dependent
manner. FEFCA significantly (*p < 0.05) attenuated the oxidative DNA damage of
mouse peritoneal macrophage cells induced by CCl4 in an in vivo assay.
Conclusion: Therefore FEFCA showed good free radical scavenging activity as well as
reduced oxidative DNA damage in mouse peritoneal macrophages in animal model.