Objective: The objective of this study was to synthesize a series of 1,3,4-thiadiazole derivative
and evaluate their possible in vitro and in vivo anticancer activity.
Method: Compounds 7a-f have been synthesized by chlorination of 2-[bis(2-hydroxyethyl)amino]-N-[5-
substituted phenyl)-1,3,4-thiadiazol-2-yl]acetohydrazide with phosphorous oxychloride and phosphorous
pentachloride. Synthesis of the targeted compounds was (7a-f) confirmed through their MP, IR, 1HNMR
and mass spectrum studies.
Results: Compounds 7a and 7d showed considerable anticancer activity against Hep-2 cell lines and,
therefore, selected for the in vitro studies and DLA cell line for the in vivo studies.
Conclusion: It may be concluded that the presence of nitro and chloro phenyl substituted 1,3,4-
thiadiazoles showed significant anticancer activity.