Title:Pharmacological Investigation and Unraveling Mechanism of Action of Jasminum sambac Flowers for Predicated Treatment of Alzheimer’s Disease
VOLUME: 14 ISSUE: 2
Author(s):Jyoti Gupta and Mayank Kulshreshtha*
Affiliation:Department of Pharmacology, School of Pharmacy, Babu Banarasi Das University, Lucknow, Department of Pharmacology, School of Pharmacy, Babu Banarasi Das University, Lucknow
Keywords:Memory impairment, Alzheimer's disease, amnesia, J. samabc, scopolamine, PAT.
Abstract:Objective: The present study was undertaken to investigate the effect of Jasminum
sambac (J. sambac) in experimental memory impairment in mice associated with Alzheimer’s disease
(AD) and tried to develop the mechanism.
Materials and Methods: Ethanolic extract of J. sambac flowers (EEJS) was administered orally
(200 and 400 mg/kg, p.o) to two groups respectively along with scopolamine, was used to induced
memory loss in swiss albino mice (1mg/kg, i.p). Models like Elevated plus maze (EPM), passive
avoidance test (PAT) and Morris water maze (MWM) test were used, biochemical estimation includes
lipid peroxidation, glutathione level, catalase and acetylcholinesterase activity with histopathology
of the brain. In-vitro cholinergic activity was also performed which may be useful to generate
the mechanism using chicken ileum as a biological indicator.
Results: It was observed that pretreatment of mice with EEJS reversed scopolamine induced amnesia,
as evidenced by a decreased in the transfer latency in the EPM task, step-down latency in
the PAT whereas decreased in escape latency in MWM test. Glutathione and Lipid peroxidation
were lowered in test groups as compared to negative control group. The extract also shows the
good cholinergic activity which proved the J. samabc acts as agonist of acetylcholine.
Conclusion: EEJS improved cognitive process by enhancing memory in different experimental
models in a dose dependent manner so that the flowers of J. samabc may be useful for the treatment
of AD in future.
