Background: Understanding the regulatory functions of the biological peptide from the
humoral central immune organ bursa of Fabricius on vaccine immune responses and antibody production
is of vital importance.
Objectives: Here we thoroughly verified the immunomodulatory functions of the new tetrapeptide
BP4 from the bursa of Fabricius on vaccine immune responses in mice and chicken immunizaiton
model, and on potential intracellular signaling during antibody production.
Method: BP4 was isolated and identified by Reverse Phase High Performance Liquid Chromatography
and matrix-assisted laser desorption ionization time of flight mass spectrometry. immunomodulatory
functions of BP4 was verified by AIV vaccine immunization on mice and chickens
regarding roles in vivo, by monitoring the impact of signalling inhibitors in hybridoma cells on
antibody production in vitro.
Results: Our investigation revealed the strong inducing roles of new isolated BP4 on immune responses
in mice immunization, the immunomodulatory effects in the immunized chicken, four potential
key intracellular signaling during antibody production in hybrdoma cells.
Conclusion: The new bursal-derived peptide BP4 was isolated and identified, and the immunomodulatory
effects on antigen-specific immune responses in vivo and in vitro were verified, suggesting
BP4 might be highly relevant to the humoral immune responses, and PI3K/Akt, p38
MAPK, NF-κB and tyrosine phosphorylation signaling might be the key activated intracellular
signaling during antibody production during BP4 stimulation, which provided a novel potential
adjuvant candidate for vaccine immunization improvement and precaution on animal epidemic