Mini-Review of the Phosphate Center Activation Strategy in Nucleoside Triphosphate Preparation

Author(s): Tomasz P. Kaczynski, Marcin K. Chmielewski*

Journal Name: Mini-Reviews in Organic Chemistry

Volume 14 , Issue 6 , 2017


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Abstract:

Background: Nucleoside triphosphates (NTPs) synthesis has recently been an area of searching for new methods and possible applications in biochemistry and medicine. However, we still lack a universal tool to synthesize NTPs easily and efficiently. For years, the most popular method has been the “one-pot, three step” conception by Ludwig with its subsequent improvements which used Yoshikawa's et al. monophosphorylation method. Further investigation of these reactions has proven many disadvantages: low yields, many byproducts and problems with purification. But they were a starting point for a whole branch of NTPs synthetic methods.

Method: Analysis of this evolution process demonstrates some correctness: using a pyrophosphate as a nucleophile and mechanism involving a cyclic intermediates at some stage. What is changing is the idea of phosphorus center activation and the resulting improvements and limitations.

Conclusion: In this paper we would like to discuss some new achievements in NTPs synthesis considering their role in changing the strategy of phosphorus center activation depending on the needs

Keywords: Nucleoside triphosphates, phosphate center activation, phosphitylation, triphosphitylation, phosphoramidite, pyrophosphate.

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Article Details

VOLUME: 14
ISSUE: 6
Year: 2017
Published on: 16 November, 2017
Page: [448 - 452]
Pages: 5
DOI: 10.2174/1570193X14666170420113635
Price: $65

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