Title:Compositional Monosaccharide Analysis of <i>Morus nigra</i> Linn by HPLC and HPCE Quantitative Determination and Comparison of Polysaccharide from <i>Morus nigra</i> Linn by HPCE and HPLC
VOLUME: 13 ISSUE: 5
Author(s):Xiao-Li Ma*, Feng Feng Song, Hui Zhang, Xie Huan and Song Ya Li
Affiliation:Central laboratory, Xinjiang Medical University, Urumqi, Department of Pharmacy, Xinjiang Medical University, Urumqi, 830011, Department of Pharmacy, Xinjiang Medical University, Urumqi, 830011, Department of Pharmacy, Xinjiang Medical University, Urumqi, 830011, Department of Pharmacy, Xinjiang Medical University, Urumqi, 830011
Keywords:Morus nigra Linn, polysaccharide, HPCE, HPLC, boric acid, linearity.
Abstract:Background: Morus nigra Linn is not only treated as health food but also medicine in Chinese
history.
Objective: Here, we have extracted and separated the heteroglycan to monosaccharides of the dry fruits.
Method: After heteroglycan being hydrolysised, then were we derivatived the monosaccharides with 1-
phenyl-3-methyl-5-pyrazolone (PMP), and then subsequently HPCE and HPLC were used to separate.
HPCE, which held an uncoated capillary (d=75µ m) and detected by PDA at 245 nm with borate buffer,
the voltage was set at 15 kV and capillary temperature was 25°. There is Yilite column (Hypersil BDS
C18 5µ m, 4.6mm× 250mm) with HPLC and detected by UV at 245 nm and capillary temperature
25°.The High Performance capillary electrophoresis method and High Performance Liquid Chromatography
method were compared on monosaccharide composition and quantitative determination of
polysaccharide from the black mulberry.
Results: Through several indicators including standard curve, precision, reproducibility, stability and
recovery rate, the result of the two methods is basically consistent while they have complementary advantages.
Conclusion: Both methods are suitable for the determination of the black mulberry polysaccharide, but
each has its own merits.
