Cloud-Point Extraction Coupled with High-Performance Liquid Chromatography for the Determination and Pharmacokinetic Study of Dipyridamole in Rat Plasma

Author(s): Jiajia Li, Yulan Li, Jingjing Liu, Min Liu, Kang Li*

Journal Name: Current Pharmaceutical Analysis

Volume 13 , Issue 5 , 2017

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Graphical Abstract:


Background: A straightforward and environment-friendly high-performance liquid chromatography/ diode array detection (HPLC/DAD) method based on cloud-point extraction (CPE) was developed for the determination of dipyridamole in rat plasma after oral administration.

Methods: The non-ionic surfactant Triton X-114 was chosen as the extract medium. Various parameters affecting the CPE efficiency were evaluated and optimized. Separation was obtained using a reversedphase Zorbax SB-C1818 column (250 × 4.6 mm, 5 µm) at 30°C with detection wavelength at 292nm

Results: Under the optimum conditions, the assay was linear over the concentration range of 0.1-20.0 µg mL-1 with the regression coefficients greater than 0.998, and the lower limit of quantification (LLOQ) was 0.1 µg mL-1. The extraction recoveries were more than 83.1%, the accuracies were within ± 9.7%, and the intra-day precision below 5.4% and inter-day precision below 12.6% in all cases.

Conclusion: The validated method is convenient, rapid and accurate for the pharmacokinetic study of dipyridamole in rat after oral administration.

Keywords: HPLC/DAD, dipyridamole, cloud-point extraction, Triton X-114, Rat plasma, pharmacokinetics.

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Article Details

Year: 2017
Published on: 07 August, 2017
Page: [481 - 487]
Pages: 7
DOI: 10.2174/1573412913666170329143017
Price: $65

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