Abstract
Introduction: We have investigated aberrant methylation of genes CDH1, RASSF1A, MLH1, N33, DAPK, expression of genes hTERT, MMP7, MMP9, BIRC5 (survivin), PTGS2, and activity of telomerase of 106 gastric tumor samples obtained intra-operatively and 53 gastric tumor samples from the same group of patients obtained endoscopically before surgery. Biopsy specimens obtained from 50 patients with chronic calculous cholecystitis were used as a control group. Together with tissue samples obtained from different sites remote to tumors, a total of 727 samples have been studied. The selected parameters comprise a system of molecular markers that can be used in both diagnostics of gastric cancer and in dynamic monitoring of patients after surgery. Special attention was paid to the use of molecular markers for the diagnostics of malignant process in the material obtained endoscopically since the efficacy of morphological diagnostics in biopsies is compromised by intratumoral heterogeneity, which may prevent reliable identification of tumor cells in the sampling. Our data indicated that certain molecular genetic events provided more sensitive yet specific markers of the tumor.
Conclusion: We demonstrated that molecular profiles detected in preoperative biopsies were confirmed by the material obtained intra-operatively. The use of endoscopic material facilitates gastric tumors pre-operative diagnostics, improving early detection of gastric cancer and potential effective treatment strategies.Keywords: Gastric cancer, Genetic markers, Molecular profiles, Biopsies, Treatment, Heterogeneous., Gastric cancer, Genetic markers, Molecular profiles, Biopsies, Treatment, Heterogeneous.
Current Genomics
Title:Implication of Gastric Cancer Molecular Genetic Markers in Surgical Practice
Volume: 18 Issue: 5
Author(s): Marina V. Nemtsova, Vladimir V. Strelnikov, Alexander S. Tanas, Igor I. Bykov, Dmitry V. Zaletaev, Viktoria V. Rudenko, Alexander I. Glukhov, Tatiana V. Kchorobrich, Yi Li, Vadim V. Tarasov, George E. Barreto and Gjumrakch Aliev*
Affiliation:
- “GALLY” International Biomedical Research Consulting LLC, San Antonio, TX 78229,USA;School of Health Sciences, University of Atlanta, Johns Creek, GA 30097, USA; Institute of Physiologically Active Compounds, Russian Academy of Sciences, Chernogolovka, 142432,Russian Federation
Keywords: Gastric cancer, Genetic markers, Molecular profiles, Biopsies, Treatment, Heterogeneous., Gastric cancer, Genetic markers, Molecular profiles, Biopsies, Treatment, Heterogeneous.
Abstract: Introduction: We have investigated aberrant methylation of genes CDH1, RASSF1A, MLH1, N33, DAPK, expression of genes hTERT, MMP7, MMP9, BIRC5 (survivin), PTGS2, and activity of telomerase of 106 gastric tumor samples obtained intra-operatively and 53 gastric tumor samples from the same group of patients obtained endoscopically before surgery. Biopsy specimens obtained from 50 patients with chronic calculous cholecystitis were used as a control group. Together with tissue samples obtained from different sites remote to tumors, a total of 727 samples have been studied. The selected parameters comprise a system of molecular markers that can be used in both diagnostics of gastric cancer and in dynamic monitoring of patients after surgery. Special attention was paid to the use of molecular markers for the diagnostics of malignant process in the material obtained endoscopically since the efficacy of morphological diagnostics in biopsies is compromised by intratumoral heterogeneity, which may prevent reliable identification of tumor cells in the sampling. Our data indicated that certain molecular genetic events provided more sensitive yet specific markers of the tumor.
Conclusion: We demonstrated that molecular profiles detected in preoperative biopsies were confirmed by the material obtained intra-operatively. The use of endoscopic material facilitates gastric tumors pre-operative diagnostics, improving early detection of gastric cancer and potential effective treatment strategies.Export Options
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Cite this article as:
Nemtsova V. Marina , Strelnikov V. Vladimir , Tanas S. Alexander , Bykov I. Igor, Zaletaev V. Dmitry , Rudenko V. Viktoria, Glukhov I. Alexander , Kchorobrich V. Tatiana , Li Yi , Tarasov V. Vadim , Barreto E. George and Aliev Gjumrakch *, Implication of Gastric Cancer Molecular Genetic Markers in Surgical Practice, Current Genomics 2017; 18 (5) . https://dx.doi.org/10.2174/1389202918666170329110021
DOI https://dx.doi.org/10.2174/1389202918666170329110021 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
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