Adipose Tissue-Derived Pericytes for Cartilage Tissue Engineering

Title:Adipose Tissue-Derived Pericytes for Cartilage Tissue Engineering

Volume: 12 Issue: 6

Author(s): Jinxin Zhang, Chunyan Du, Weimin Guo, Pan Li, Shuyun Liu, Zhiguo Yuan, Jianhua Yang, Xun Sun, Heyong Yin, Quanyi Guo* Chenfu Zhou*

Affiliation:

• Institute of Orthopaedics, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853,China

• Institute of Orthopaedics, First Affiliated Hospital of Jiamusi University, Jiamusi 154003,Heilongjiang Province,China

Keywords: Pericytes, cartilage tissue engineering, CD146, adipose tissue-derived stromal vascular fraction (AT-SVF), magnetic- activated cell sorting (MACS), adipose-derived stem cells (ADSCs), chondrogenic progenitor cells (CPCs).

Abstract: Background: Mesenchymal stem cells (MSCs) represent a promising alternative source for cartilage tissue engineering. However, MSC culture is labor-intensive, so these cells cannot be applied immediately to regenerate cartilage for clinical purposes. Risks during the ex vivo expansion of MSCs, such as infection and immunogenicity, can be a bottleneck in their use in clinical tissue engineering. As a novel stem cell source, pericytes are generally considered to be the origin of MSCs. Pericytes do not have to undergo time-consuming ex vivo expansion because they are uncultured cells. Adipose tissue is another optimal stem cell reservoir. Because adipose tissue is well vascularized, a considerable number of pericytes are located around blood vessels in this accessible and dispensable tissue, and autologous pericytes can be applied immediately for cartilage regeneration.

Objective: Thus, we suggest that adipose tissue-derived pericytes are promising seed cells for cartilage regeneration.

Conclusion: Many studies have been performed to develop isolation methods for the adipose tissuederived stromal vascular fraction (AT-SVF) using lipoaspiration and sorting pericytes from AT-SVF. These methods are useful for sorting a large number of viable pericytes for clinical therapy after being combined with automatic isolation using an SVF device and automatic magnetic-activated cell sorting. These tools should help to develop one-step surgery for repairing cartilage damage. However, the use of adipose tissue-derived pericytes as a cell source for cartilage tissue engineering has not drawn sufficient attention and preclinical studies are needed to improve cell purity, to increase sorting efficiency, and to assess safety issues of clinical applications.

Cite this article as:

Zhang Jinxin, Du Chunyan , Guo Weimin, Li Pan , Liu Shuyun , Yuan Zhiguo, Yang Jianhua , Sun Xun , Yin Heyong, Guo Quanyi *, Zhou Chenfu *, Adipose Tissue-Derived Pericytes for Cartilage Tissue Engineering, Current Stem Cell Research & Therapy 2017; 12 (6) . https://dx.doi.org/10.2174/1574888X12666170321111211

DOI https://dx.doi.org/10.2174/1574888X12666170321111211	Print ISSN 1574-888X
Publisher Name Bentham Science Publisher	Online ISSN 2212-3946