Title:Proteomic Changes Between <i>Populus</i> Allotriploids and Diploids Revealed Using an iTRAQ-based Quantitative Approach
VOLUME: 14 ISSUE: 3
Author(s):Yi Wang, Yun Li, Yujing Suo, Yu Min and Xiangyang Kang*
Affiliation:National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing 100083, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing 100083, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing 100083, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing 100083, Beijing Forestry University, No. 35, Qinghua East Road, Beijing 100083
Keywords:iTRAQ, Populus, allotriploid, diploids, proteome, polyploid.
Abstract:Background: Polyploid breeding is a powerful approach for Populus genetic improvement
because polyploid trees have valuable characteristics, including better timber quality and a
higher degree of stress resistance compared with their full-sib diploids. However, the genetic
mechanism underlying this phenomenon remains unknown.
Objective: To better understand the proteomic changes between Populus allotriploids and diploids,
we examined the proteomic profiles of allotriploid and diploid Populus by iTRAQ labeling coupled
with two-dimensional liquid chromatography and MALDI-TOF/TOF mass spectrometry
(MS).
Method: iTRAQ labeling coupled with two-dimensional liquid chromatography and MALDITOF/
TOF mass spectrometry (MS).
Results: Between the
Populus allotriploid and the full-sib diploid, 932 differentially expressed
proteins (DEPs) were identified. These DEPs were primarily involved in stress, defense, transportation,
transcriptional and/or translational modification, and energy production. The pathway
analysis indicated that most of the DEPs were implicated in carbohydrate transport and metabolism,
nitrogen metabolism and glycolysis, and the ribosome assembly pathway. These data suggest
high protein divergence between
Populus allotriploids and diploids, and rapid changes during
hybridization.
Conclusion: The results provide new data for further understanding of the mechanisms of polyploid
trees that generally display increased height growth compared with their full-sib diploids.
