Background: Bergenin, a compound derived from gallic acid, is a secondary metabolite
of the plant Peltophorum dubium (Spreng.) Taub.
Objective: In this study, we aimed to characterize the ability of bergenin to eliminate the radicals
in non-biological systems.
Methods: We evaluated bergenin’s ability to protect erythrocytes from oxidative damage in a biological
system. We have elucidated bergenin structure using nuclear magnetic resonance, X-ray
diffraction, Fourier transform infrared spectroscopy, and differential scanning calorimetry. We
then evaluated its antioxidant capacity in vitro against DPPH•
, hydroxyl radicals, and nitric
oxide, and determined its ability to transfer electrons owing to its reduction potential and ability
to chelate iron. We also evaluated its protective capacity against oxidative damage produced by
AAPH in erythrocytes, its hemolytic properties, its ability to inhibit hemolysis, and its ability to
maintain intracellular reduced glutathione homeostasis.
Results: Bergenin concentrations between 0.1 and 3mM significantly (p < 0.05) and dose dependently
decreased formation of ABTS•+
, nitrite ions, OH•
, reduced formation ferricyanide,
complex, inhibited AAPH-induced oxidative hemolysis of erythrocytes, raised GSH
levels in the presence of AAPH, inhibited AAPH-induced lipid peroxidation in erythrocytes.
Conclusion: Bergenin may represent a novel alternative antioxidant, with potential applications in
various industries, including drugs, cosmetics, and foods.