Quality Assurance of Immunohistochemistry for Breast Cancers using a Whole-slide Imaging System, Specified Software and Cell Lines
Pp. 477-480 (4)
Miho Yoshida-Tanaka, Junichi Sakane, Yoshiko Kimura, Yoshimi Shitakubo, Kazuya Kuraoka and Kiyomi Taniyama
An auto-stainer (AS), whole-slide imaging system (WSI), and computer
software were used as quality assurance tools for breast cancer immunohistochemistry
Cell lines, such as MCF-7 for estrogen receptors and progesterone receptors, and SKBR-
3 for Topo2a, were co-stained with samples as IHC control.
Combinatory usage of AS, WSI, computer software and cell lines can reduce deviation
in each IHC step and is beneficial for IHC quality assurance.
Auto-analysis, Breast cancer, Immunohistochemistry, Quality
assurance, Whole slide image.
National Hospital Organization, Kure Medical Center and Cancer Center, 3-1, Aoyama-cho, Kure 737-0023, Japan.