Influence of Endoscopic Stent Insertion on Detection of Circulating Tumor Cells from Obstructing Colon Cancer
Pp. 80-86 (7)
Shinya Yamashita, Masahiro Tanemura, Toshio Kuwai, Yosuke Shimizu, Harumi Tominaga and Nobutaka Hatanaka
Objective: Self-expanding metallic stents (SEMS) have been employed as a palliative
treatment for patients with obstructive colorectal cancer or as bridge to surgery for
potentially resectable colorectal cancer (CRC). However, concerns have been raised
about whether shear forces that act on the tumor during expansion of the stent may
result in a release of cancer cells into the circulation (i.e., CTCs). This study was
conducted to determine whether insertion of colonic stents results in increased levels of
Methods: Between October 2013 and November 2013, three patients who underwent
colonic stent insertion for obstructing colorectal cancer were studied. To detect viable
CTCs of CRC, we employed a TelomeScanF35 detection system, which was
constructed with a GFP-expressing attenuated adenovirus, in which the telomerase
promoter regulates viral replication. 7.5ml of peripheral blood samples were obtained
before and after stent insertion and after operation.
Results: Three patients were inserted SEMS and performed laparoscopic surgery. In one of these 3 patients, CTCs increased after stent insertion and continued in
expression after the operation.
Conclusions: This study demonstrated that endoscopic stent insertion results in
dissemination of tumor cells into the peripheral circulation. Although oncological
consequences were not examined in this small population, viable CTC detected by
TelomeScanF35 may represent a suitable prognostic marker to stratify the risk of
patients with distant metastasis following stent insertion for obstructing colon cancer.
Bridge to surgery, Circulating tumor cells, Obstructing colon cancer,
Self-expanding metallic stent, TelomeScan F35.
Department of Surgery, Osaka Police Hospital, 10-31 Kitayama-cho, Tennouji-ku , Osaka 543-0035, Japan.