Aim and Objective: Due to the common ethnopharmacological used or scientifically
examined biochemical properties, Elaeagnaceae family, Elaeagnus umbellate (Thunb.) (EU, Guz
yemisi) was worth investigating.
Materials and Methods: In this investigation, we revealed antioxidant, antiproliferative and enzyme
inhibition activities of the water, methanol, ethanol, acetone, ethyl acetate and hexane extracts of
EU as well as the contents of their phenolic, flavonoid, anthocyanin, ascorbic acid, lycopene and β-
carotene. The antioxidant activity was screened by total antioxidant (phosphomolybdenum),
inhibition of linoleic acid peroxidation, reducing power, 2-deoxyribose degradation assay, H2O2
scavenging and metal chelating activities of the samples were tested in vitro. Additionally, the
scavenging activities of the extracts were determined against 1,1-diphenyl-2-picrylhydrazyl
(DPPH˙), 2,2-azino-bis(3-ethylbenzothiazloine-6-sulfonicacid (ABTS˙+), superoxide anion and
peroxide radicals. The samples were determined for their inhibitory activities against urease, acetylcholinesterase
(AChE) and butyrylcholinesterase (BuChE). In vitro, antiproliferative activities of six
different extracts were tested using the xCELLigence system against HeLa and HT29 cell lines.
Results: The antioxidant activities of the extracts were found higher than standard antioxidants. The
water extracts of fruit and leaf showed the best antioxidant activity. In inhibition assays of urease,
AChE and BuChE, all extracts exhibited remarkable inhibition potential. Ethyl acetate extracts,
especially, showed better inhibition capacity. It was found that the antioxidant activities of the
extracts presented consistently with their chemical contents. The antiproliferative activities of leaf
extracts were more effective than the fruit extracts. The chromatographic methods were applied to
the different solvents to analyses phenolic secondery metabolites. It was found that fumaric acid, 4-
hydroxybenzoic acid, rutin and quercetin-3-β-D-glucoside, neohesperidin, hesperidin determined to
have higher contents all the extracts.
Conclusion: EU can be suggested as a potential natural source of antioxidants appropriate for
utilization in nutritional/pharmaceutical fields.