Background: The investigation of the cellular components, their interactions and related functions constitute
the major conditions in order to understand the cell as an integrated system. More specifically, the Protein-Protein
Interactions and the obtained networks are very important in the majority of biological functions and processes, while
most of the proteins appear to activate their functionalities through their interaction.
Methods: Our in depth review analysis, include Sixty-five peer-reviewed research and review studies from several
bibliographic databases. The most significant components were fully described, filtered, combined and analyzed in
order to provide documented proofs on the Protein-Protein Interaction Network' applications in biomedicine.
Results: The Protein-Protein Interaction Network' alignment and mapping give the opportunity of further knowledge
extraction concerning the evolutionary relationships between the species through conserved pathways and protein
complexes. Additionally, Protein-Protein Interaction Network information has been demonstrated to be able to predict
functionally orthologous proteins within sequence homology clusters. Our review analysis concluded that, while Protein-
Protein Interaction was used to be characterized just by their large and plain interacting surfaces, they were considered
inapplicable for drug discovery studies for a long time.
Conclusion: The present review explores multiple technologies implicated in Protein-Protein Interaction Networks,
implicating their potential role in drug discovery mechanisms.