Objective: The purpose of the study was to formulate and evaluate thiolated
chitosan based nanoparticulate mucoadhesive ophthalmic in situ gelling system of prulifloxacin
for bacterial keratoconjunctivitis.
Method: Low molecular weight chitosan was modified to thiolated chitosan in the laboratory
and confirmed with FTIR and DSC studies. Thiolated chitosan nanoparticles were
prepared and incorporated in a polymeric sol-gel system containing prulifloxacin for the
ophthalmic drug delivery. The prepared formulation was characterized for the various
physicochemical properties i.e., particle size, zeta potential, gelation time, mucoadhesion
strength study and the optimized formulation was characterized for in vitro drug release
study, ocular irritation study, antimicrobial efficacy study and stability study etc.
Results: Gelation pH was found to be 7.2±0.2. The acquired SEM images of the optimized
NPs showed good mean particle size below 16 nm. Drug entrapment efficiency was found
to be more than 80%. PFL-TCS loaded NPs sol-gel system showed sustain release for a
period of 12 hours. It was also confirmed that the formulation was non-irritant to the eye.
Conclusion: The developed formulation was found to be a better alternative to the conventional