In nano drug formulations the mechanism of release is a critical process to recognize controlled
and targeted drug delivery systems. In order to gain high bioavailability and specificity from
the drug to reach its therapeutic goal, the active substance must be loaded into the nanoparticles efficiently.
Therefore, the amount in biological fluids or tissues and the remaining amount in nano carriers
are very important parameters to understand the potential of the nano drug delivery systems. For
this aim, suitable and validated quantitation methods are required to determine released drug concentrations
from nano pharmaceutical formulations.
HPLC (High Performance Liquid Chromatography) is one of the most common techniques used for
determination of released drug content out of nano drug formulations, in different physical conditions,
over different periods of time. Since there are many types of HPLC methods depending on detector
and column types, it is a challenge for the researchers to choose a suitable method that is simple,
fast and validated HPLC techniques for their nano drug delivery systems.
This review’s goal is to compare HPLC methods that are currently used in different nano drug delivery
systems in order to provide detailed and useful information for researchers.