Title:Rapid Biosynthesis of Gold Nanoparticles Using Aqueous-ethanoic Leaf Extract of Heartleaf Moonseed: Characterization and Effect of pH on its Synthesis
VOLUME: 2 ISSUE: 1
Author(s):Nand K. Roy, Javadi Monisha, Ganesan Padmavathi, Anusmita Das, Sudeshna Gupta, Elancheran Ramakrishnan, Jibon Kotoky and Ajaikumar B. Kunnumakkara*
Affiliation:Cancer Biology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam-781039, Cancer Biology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam-781039, Cancer Biology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam-781039, Cancer Biology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam-781039, Cancer Biology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam-781039, Division of Life Sciences, Institute of Advanced Study in Science and Technology (IASST), Paschim Boragaon, Guwahati, Assam-781035, Division of Life Sciences, Institute of Advanced Study in Science and Technology (IASST), Paschim Boragaon, Guwahati, Assam-781035, Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati, Guwahati, Assam-781039
Keywords:Gold nanoparticles, medicinal plant, zeta potential, X-ray diffraction, fourier transforms infrared spectroscopy,
transmission electron microscopy.
Abstract:Background: Gold nanoparticles (GNPs) are of tremendous interest due to their wide application
range in the field of therapeutics and diagnostics. However, its potential is limited by its
mode of synthesis utilizing different chemicals which are costly, hazardous and often labor-intensive.
Nevertheless, the GNPs synthesized through green methods by using plant extracts can overcome
these limitations and have wider acceptance.
Objective: The present study intends to synthesize and characterize the GNPs by exploiting the
bioreducing properties of Heartleaf moonseed leaf extract (HMLE), a well-known medicinal plant.
Method: Different volume fractions (1-6%) of aqueous- ethanoic extract of Heartleaf moon seed were
used for GNPs synthesis via microwave heating for a minute. The synthesis of GNPs was confirmed
by UV-visible spectroscopy and characterized by Zeta potential measurement, Fourier Transform Infra-
red Spectroscopy (FTIR), X- ray Diffraction studies (XRD) and Transmission Electron Microscopy
(TEM).
Results: UV-visible spectroscopy confirmed GNP formation giving a characteristic peak at 560 nm.
Zeta potentiometer showed the charge of GNPs as -30.3mV. XRD pattern of gold that is (111), (200),
(220) and (311) was obtained due to Bragg reflections corresponding to different lattice planes of
GNPs. FTIR analysis indicated the surface adsorption of biomolecules containing asymmetric and
symmetric CH2 stretch bands. TEM image showed different sizes of GNPs with an average of 12 ±
3.7 nm and shape such as polygonal, cylindrical and prism-shaped GNPs.
Conclusion: This method avoids the use of toxic chemicals; hence possesses immense prospect in
vivid biomedical applications.
