Title:Agar/Chitosan IPN Thin Hydrogel Films with Antimicrobial and Antioxidant Properties for Potential Dressing Applications
VOLUME: 1 ISSUE: 1
Author(s):Nurettin Sahiner*, Selin Sagbas, Mehtap Sahiner, Coskun Silan, Nahit Aktas and Mustafa Turk
Affiliation:Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University, Terzioglu Campus, 17100 Canakkale, Faculty of Science & Arts, Chemistry Department, Ege University, Engineering Faculty, Leather Engineering Department, 35100 Bornova, Izmir, School of Medicine, Department Pharmacology, Canakkale Onsekiz Mart University, Terzioglu Campus, 17100, Chemical Engineering Department, Yuzuncu Yil University, Campus, Van, 65080, Bioengineering Department, Engineering Faculty, Kirikkale University, Yahsihan, 71450, Kirikkale
Keywords:Agar/chitosan thin hydrogel film, antimicrobial, antioxidant, interpenetrating polymer network (IPN), wound dressing
IPN, rosmarinic acid release.
Abstract:Background: Polymeric films derived from natural sources biopolymers are promising materials
as wound dressing materials due to their biodegradable, biocompatible, non-toxic natures.
Objective: To demonstrate the preparation of agar/chitosan thin hydrogel films for potential wound
dressing applications.
Method: The swelling and moisture content of hydrogel films at different pHs were determined. Also,
their antioxidant properties via total phenol content, and ABTS+ scavenging assay methods were investigated.
Antimicrobial properties against various bacteria and the cytotoxicity of the prepared hydrogel
films were determined by means of WST-1 assay against L929 fibroblast cells.
Results: The maximum swelling values of RA containing agar/0.17chitosan thin hydrogel films were
determined as 255 ± 88%, 209 ± 41%, and 262 ± 31% at pH 5.4, 7.4, and 9, respectively. Also, the
moisture contents at these pHs were also found as 79 ± 0.3%, 76 ± 1.3%, and 82 ± 0.9%, respectively.
RA is found to enhance the antioxidant capacity prepared films with 17.99 mM trolox equivalencyg-1
antioxidant capacity. Agar/0.33chi hydrogel film and its HCl treated forms are found effectively antimicrobial
materials against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Candida albicans
strains with 10-5 mg mL-1 of MIC values. Moreover, agar and agar/0.33chi hydrogels were found
to use as safe, whereas agar/0.17chi/0.083RA hydrogel films were demonstrated good biocompatibility
against L929 fibroblast cells.
Conclusion: Soft and flexible structure of agar/chi based hydrogel films afford great potential for open
wounds as covering martials with high swelling ability and moisture content. These with RA materials
possess good antioxidant properties, and effectively antimicrobial properties and found biocompatible
against L929 fibroblast cells.
