Agar/Chitosan IPN Thin Hydrogel Films with Antimicrobial and Antioxidant Properties for Potential Dressing Applications

Author(s): Nurettin Sahiner*, Selin Sagbas, Mehtap Sahiner, Coskun Silan, Nahit Aktas, Mustafa Turk

Journal Name: Current Applied Polymer Science

Volume 1 , Issue 1 , 2017

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Graphical Abstract:


Background: Polymeric films derived from natural sources biopolymers are promising materials as wound dressing materials due to their biodegradable, biocompatible, non-toxic natures.

Objective: To demonstrate the preparation of agar/chitosan thin hydrogel films for potential wound dressing applications.

Method: The swelling and moisture content of hydrogel films at different pHs were determined. Also, their antioxidant properties via total phenol content, and ABTS+ scavenging assay methods were investigated. Antimicrobial properties against various bacteria and the cytotoxicity of the prepared hydrogel films were determined by means of WST-1 assay against L929 fibroblast cells.

Results: The maximum swelling values of RA containing agar/0.17chitosan thin hydrogel films were determined as 255 ± 88%, 209 ± 41%, and 262 ± 31% at pH 5.4, 7.4, and 9, respectively. Also, the moisture contents at these pHs were also found as 79 ± 0.3%, 76 ± 1.3%, and 82 ± 0.9%, respectively. RA is found to enhance the antioxidant capacity prepared films with 17.99 mM trolox equivalencyg-1 antioxidant capacity. Agar/0.33chi hydrogel film and its HCl treated forms are found effectively antimicrobial materials against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Candida albicans strains with 10-5 mg mL-1 of MIC values. Moreover, agar and agar/0.33chi hydrogels were found to use as safe, whereas agar/0.17chi/0.083RA hydrogel films were demonstrated good biocompatibility against L929 fibroblast cells.

Conclusion: Soft and flexible structure of agar/chi based hydrogel films afford great potential for open wounds as covering martials with high swelling ability and moisture content. These with RA materials possess good antioxidant properties, and effectively antimicrobial properties and found biocompatible against L929 fibroblast cells.

Keywords: Agar/chitosan thin hydrogel film, antimicrobial, antioxidant, interpenetrating polymer network (IPN), wound dressing IPN, rosmarinic acid release.

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Article Details

Year: 2017
Published on: 11 May, 2017
Page: [52 - 62]
Pages: 11
DOI: 10.2174/2452271601666161209153207

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