Objective: Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver
disease and becoming an important public health concern in the world. However, the molecular
mechanism of the pathogenesis in NAFLD is still unclear.
Method: In this study, in vitro model of NAFLD was established and differential expression of proteins
in this cellular model was investigated by using proteomic technique.
Result: By using two-dimensional polyacrylamide gel electrophoresis, approximately 650 proteins
were separated and sixteen were found to be significantly affected by oleic acid compared to the untreated
control. Among them, eight were upregulated and other eight were downregulated. These
proteins were successfully identified with peptide mass fingerprinting using matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) after in-gel trypsin digestion.
The results showed that these proteins are involved in transcription regulation (53.4%), metabolic
process (13.3%), cytoskeleton (20%), and cellular signaling transduction (13.3%), respectively.
Conclusion: It is suggested that the pathogenesis of NAFLD is associated with the differential expression
of some functional proteins. These proteins may provide potential targets for future mechanistic
studies concerning the development and prevention of NAFLD.