Background: Modelling and analysis of metabolic networks can be carried out with different
levels of granularity, ranging from structural (topological) analysis to constraint-based methods to
kinetic modelling. The increasing number of genome-scale metabolic reconstructions and the growing
importance of metabolic modelling intensify the need for integrated approaches allowing the application
of different analysis methods.
Objective: There is no well-organized and freely accessible code package or specific toolbox/plugin
available for the reconstruction of metabolite- and enzyme-centric networks. So, we have developed a
toolbox which could be able to perform such reconstructions.
Method: We have developed the SCAN-toolbox, an Add-on to the widely used COBRA framework for
constraint-based modelling. SCAN is an open-source MATLAB/Octave toolbox which extends the
COBRA framework and provides functions to prepare different metabolite- and enzyme-centric
networks that could also be imported in other structural analysis softwares, such as Cytoscape.
Results: We have applied the SCAN-toolbox to four metabolic networks to demonstrate its
Conclusion: SCAN extends the COBRA framework towards structural analysis of large metabolic
networks, including the reconstruction of undirected and directed metabolite- as well as enzyme-centric