Title:Determination of Imatinib and CGP74588 in Rat Plasma by HPLC and Its Application to a Pharmacokinetic Study
VOLUME: 14 ISSUE: 2
Author(s):Jiamiao Lin, Na Ying, Yanli Wu and Gaotong Lin*
Affiliation:Department of Pharmacy, Shulan (Hangzhou) Hospital & Zhejiang University International Hospital, Hangzhou, Department of Pharmacy, Taizhou Cancer Hospital, Zhejiang Wenling, Department of Pharmacy, Shulan (Hangzhou) Hospital & Zhejiang University International Hospital, Hangzhou, Department of Pharmacy, Taizhou Cancer Hospital, Zhejiang Wenling
Keywords:HPLC, rat, pharmacokinetics, imatinib, CGP74588, N-desmethyl imatinib.
Abstract:Objective: A simple, reliable and specific method of HPLC which was performed on a reversed-
phase Agilent SB column was developed for the determination of imatinib and CGP74588.
Method: The mobile phase comprised water-acetonitrile-0.1% trifluoroacetic acid (59:21:20, v/v/v)
flowed at the rate of 1 mL/min at 40°C. The detection wavelength was 278nm.
Results: Sensitivity has been illustrated in this method, and a good linear relationship was observed
between 0.1-20.00 µg/mL for imatinib with r2=0.9994 and 0.01-2.00 µg/mL for CGP74588 with
r2=0.9998 in rat plasma. The relative recoveries of imatinib and CGP74588 were all more than 93%,
whilst the absolute recoveries of imatinib and CGP74588 were all more than 75%. Both intra- and
inter-day precisions were all less than 4.5% for imatinib and 4.74% for GCP74588. The limits of quantification
of these analytic methods were 0.1 µg/mL for imatinib and 0.01 µg/mL for CGP74588.
Conclusion: The method was suitable for studying the pharmacokinetic parameters of imatinib and
GCP74588 and routine analysis.
