Title:In vitro Antioxidant Activity of Passion Fruit (Passiflora alata) Extract by Different Kinds of Treatment on Rat Liver
VOLUME: 14 ISSUE: 1
Author(s):Niara S. Medeiros, Daniela C. Almeida, Juliana D. Lima, Mariane Wohlemberg, Fernanda Machado, Marielle Massolini, Fabiana Agostini, Claudia Funchal, Sabrina Bortolazzi and Caroline Dani*
Affiliation:Research Center, Centro Universitario Metodista IPA, Porto Alegre, Research Center, Centro Universitario Metodista IPA, Porto Alegre, Research Center, Centro Universitario Metodista IPA, Porto Alegre, Research Center, Centro Universitario Metodista IPA, Porto Alegre, Research Center, Centro Universitario Metodista IPA, Porto Alegre, Research Center, Centro Universitario Metodista IPA, Porto Alegre, Institute of Biotechnology, Universidade de Caxias do Sul, Caxias do Sul, Research Center, Centro Universitario Metodista IPA, Porto Alegre, Institute of Biotechnology, Universidade de Caxias do Sul, Caxias do Sul, Research Center, Centro Universitario Metodista IPA, Porto Alegre
Keywords:Passiflora alata, passion fruit, sodium azide, oxidative stress, polyphenols.
Abstract:Background: Passion fruit is known to be rich in flavonoids and can be an important antioxidant,
preventing the oxidative damage. The objective of this study was first to evaluate the antioxidant
activity of Passiflora alata fruit extract in the liver of Wistar rats and second to compare the efficacy
of pretreatment or co-treatment of this extract.
Method: Antioxidant activity of Passiflora alata fruit extract was analyzed by lipid peroxidation
(TBARS), protein oxidation (carbonyl), Sulfhydryl levels and enzymatic activity of superoxide dismutase
(SOD) and of catalase (CAT) against sodium azide damage induced in the liver of Wistar rats.
Results: The pre-treatment extract reduced and/or prevented the lipid peroxidation and protein oxidative
damage caused by sodium azide. The co-treatment didn’t show any protection at the level of protein
oxidation damage, in this case an increase of damage was observed. Sulfhydryl levels increased in
all groups. The SOD activity was not significantly different between groups, but the CAT activity increased
in the group with co-treatment.
Conclusion: Pre-treatment with passion fruit (Passiflora alata) can be an alternative in reducing oxidative
damage in the liver.
