Background: Phytol (3,7,11,15-tetramethylhexadec-2-en-1-ol; PHY), the alcoholic diterpenoid
is particularly interesting due to its diverse activities found in literature. This study evaluated in vitro
and in vivo antioxidant capacity of PHY.
Methods: We conducted DPPH• (2,2-diphenyl-1-picrylhydrazyl) and ABTS•+ (2,2'-azino-bis(3-
ethylbenzthiazoline-6-sulphonic acid)) radical scavenging tests as in vitro, while Saccharomyces cerevisiae
test as in vivo. For in vitro tests, trolox and for in vivo test hydrogen peroxide (H2O2) were taken
as standard and stressor, respectively. Additionally, we measured the superoxide dismutase (SOD), catalase
(CAT), reduced glutathione (GSH), lipid peroxidation (LP) and nitrite (NO2
-) contents in mouse
hippocampus taking 0.05% Tween 80 dissolved in 0.9% saline (0.25 ml) and ascorbic acid (250 mg/kg;
AA) as vehicle and standard, respectively. PHY was administered at doses 25, 50 and 75 mg/kg. In the
latter case, all the treatments were administered via intraperitoneal (i.p.) route.
Results: PHY at 7.2 μg/ml exhibited 59.89 ± 0.73% and 62.79 ± 1.99% scavenging capacity of DPPH•
and ABTS•+, respectively. In S. cerevisiae strains, PHY showed prominent protective effects. Moreover,
in Swiss mouse hippocampus; PHY reduced the LP and NO2
- contents, while increased in GSH, SOD
and CAT activities.
Conclusion: PHY exerted antioxidant potential in our current non- and preclinical test systems and can
be a good candidate for the development of treatments of oxidative stress mediated diseases.