Background: Glioblastoma has been reckoned as the prime cause of death due to brain tumours,
being the most invasive and lethal. Available treatment options, i.e. surgery, radiotherapy, chemotherapy
and targeted therapies are not effective in improving prognosis, so an alternate therapy is insistent. Plant
based drugs are efficient due to their synergistic action, multi-targeted approach and least side effects.
Methods: The anti-tumorous potential of Nardostachys jatamansi rhizome extract (NJRE) on U87
MG cell line was evaluated through various in vitro and in silico bio-analytical tools.
Results: NJRE had a strong anti-proliferative effect on U87 MG cells, Its IC50
30.59±3.4 and 28.39±2.9 μg/mL, respectively after 24, 48 and 72 h. NJRE at 30 μg/mL induced DNA
fragmentation, indicating apoptosis, early apoptosis began in the cells at 20 μg/mL, whereas higher
doses exhibited late apoptosis as revealed by dual fluorescence staining. NJRE at 60 and 80 μg /mL
caused a G0
arrest and at 20 and 40 μg/mL showed excessive nucleation and mitotic catastrophe in
the cells. Immuno-blotting validated the apoptotic mode of cell death through intrinsic pathway.
NJRE was harmless to normal cells. In silico docking of NJRE marker compounds: oroselol,
jatamansinol, nardostachysin, jatamansinone and nardosinone have revealed their synergistic and
multi-targeted interactions with Vestigial endothelial growth factor receptor 2 (VEGFR2), Cyclin dependent
kinase 2 (CDK2), B-cell lymphoma 2 (BCL2) and Epidermal growth factor receptor (EGFR).
Conclusion: A strong dose specific and time dependent anti-tumorous potential of NJRE on U87 MG
cells was seen. The extract can be used for the development of safe and multi-targeted therapy to
manage glioblastoma, which has not been reported earlier.