Title:The Effects of Hydroalcoholic Extract of Pinus eldarica on Hippocampal Tissue Oxidative Damage in Pentylenetetrazole-Induced Seizures in Rat
VOLUME: 13 ISSUE: 1
Author(s):Rahimeh Bargi, Fereshteh Asgharzadehyazdi, Farimah Beheshti, S. Mojatab Mousavi, Shabnam Vafadar Langehbiz, Hassan Rakhshandeh, Hamid Reza Sadeghnia and Mahmoud Hosseini
Affiliation:Neurocognitive Research Center, School of Medicine, Azadi Square, Mashhad, Postal Code: 9177948564
Keywords:Pinus eldarica, pentylenetetrazole, seizures, oxidative stress, hippocampus.
Abstract:Background: Brain tissues oxidative damage has been proposed to occur as a result of
epileptic seizures. With respect to the antioxidant effects of Pinus eldarica (P. eldarica), the impacts
of a hydroalcoholic extract of the plant on the brain tissues oxidative damage taking after seizures
induced by Pentylenetetrazole (PTZ) was investigated in rats.
Methods: The rats were divided into 6 groups and treated: (1) Control(saline); (2) PTZ (100 mg/kg,
i.p.), [3-6] four doses including 20, 50, 200 and 500 mg/kg of P. eldarica extract (Ext 20, Ext 50, Ext
200, Ext 500) 30 min before PTZ injection. Latencies to the first Minimal Clonic (MCS) and Generalized
Tonic-Clonic (GTCS) seizures were recorded. The hippocampal tissues were then collected
for biochemical measurements.
Results: The extract non-significantly postponed both the MCS and GTCS onsets. PTZ- induced seizure
increased Malondialdehyde (MDA) (P<0.001) concentration while, diminished thiol contents
(P<0.01) of hippocampal tissues. Pretreatment with 200 mg of the extract diminished MDA level in
the hippocampal tissues compared to the PTZ group (P<0.001). Both 50 and 200 mg/kg of the extract
improved thiol concentration in the hippocampal tissues (p<0.01).
Conclusion: The present study demonstrated that the extract of P. eldarica possess protective effects
against hippocampal tissues oxidative damage in PTZ- induced seizure model.
