Background: Many species of the genus Vitex from the Verbenaceae family are used in traditional
medicine for the treatment of various medical conditions such as depression, venereal diseases,
malaria, asthma, allergy, wounds, skin diseases, snake bite and body pains. One of them, Vitex agnuscastus
L. has been used to treat pain, swelling, inflammation, headaches, rheumatism, and sexual dysfunction.
Methods: The analgesic and anti-inflammaotry effects of essential oil extracts from Vitex agnus-castus L
were evaluated employing various standard experimental test models. Acetic acid was used to induce
writhes in mice and inflammation of paw edema. The analgesic activity of seeds essential oil at a dose of
150mg / kg gave a percentage of inhibition very near to diclofenac (40.13% and 40.41%, respectively).
The essential oils show an anti-inflammatory activity in the test of the edema.
Results: The evaluation of the percentage of inhibition indicates that the essential oil of Vitex agnuscastus
L. seeds possess anti-inflammatory activity (150 mg/kg) nearby and sometimes better -during the
time-than diclofenac (61,04% - 50% at 30 min; 62,04% - 83,33% at 60 min and 91,05% - 90,91% at 120
Conclusion: The results of this research indicated that Vitex agnus-castus L. essential oil of seeds remarkably
inhibited inflammation and could explain the traditional usage of this plant.