Correlations Between Experimentally-Determined Melting Temperatures and GC-Content for Short DNA Strands

Author(s): Dan Tulpan*, Roberto Montemanni, Derek H. Smith.

Journal Name: Current Bioinformatics

Volume 12 , Issue 4 , 2017

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Abstract:

Background: The hybridization stability of single and double stranded DNA sequences has been studied extensively and its impact on bio-computing, bio-sensing and bio-quantification technologies such as microarrays, Real-time PCR and DNA sequencing is significant. In many bioinformatics applications DNA duplex hybridization is traditionally estimated using GC-content and melting temperature calculations based on the sequence base composition.

Objective: In this study we explore the equivalence of the two approaches when estimating DNA sequence hybridization and we show that GC-content is a far from perfect predictor of DNA strand hybridization strength compared to experimentally-determined melting temperatures.

Method: To test the assumption that DNA GC-content is a good indicator of its melting temperature, we formulate a research hypothesis and we apply the Pearson product-moment correlation statistical model to measure the strength of a linear association between the GC-content and melting temperatures.

Results: We built a manually curated set of 373 experimental data points collected from 21 publications, each point representing a DNA strand with length between 4 and 35 nucleotides and its corresponding experimentally determined melting temperature measured under specific sequence and salt concentrations. For each data point we calculated the corresponding GC-content and we separated the set into 12 subsets to minimize the variability of experimental conditions.

Conclusion: Based on calculated Pearson product-moment correlation coefficients we conclude that GC-content only seldom correlates well with experimentally determined melting temperatures and thus it is not a strictly necessary constraint when used to control the uniformity of DNA strands.

Keywords: DNA sequence, GC-content, hybridization, melting temperature, oligonucleotides, Pearson correlation.

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Article Details

VOLUME: 12
ISSUE: 4
Year: 2017
Page: [296 - 302]
Pages: 7
DOI: 10.2174/1574893611666161008194920
Price: $58

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