Background: A growing evidence indicates that marine sponge Phyllospongia sp. is one
of rich sources of 20, 24-bishomoscalarane sesterterpenes with potent biological activities. In order
to search more bioactive 20, 24-bishomoscalarane sesterterpenes for new drug discovery, chemical
investigation was carried out on an Indonesian marine sponge P. papyrecea.
Methods: Bioassay-guided fractionation was carried out on its dichloromethane extract. And nine compounds
were purified and isolated using HPLC. Their chemical structures were determined by a combination
of spectroscopic and spectrometric data, including 1D-, 2D-NMR and HRESI-MS. Their cytotoxic
activities were performed on three human tumor cell lines A549, MCF-7 and HeLa using the
Results: One new 20, 24-bishomoscalarane sesterterpene, phyllactone H (9), was isolated and elucidated
together with phyllactones A-B (1-2) and D-G (3-6), 12α, 24-dihydroxy-20, 24-dimethyl-15, 17-
scalaradien-25, 24-olides (7-8). Compounds 1 and 2, 3 and 4, 5 and 6, 7 and 8 were C-24 anomers and
inseparable mixtures, respectively. The 1H and 13C-NMR data for 7/8 were firstly reported in this paper.
Conclusion: Compounds 1-9 possessed in vitro moderate cytotoxicities against A549, MCF-7 and
HeLa cells with IC50 values of less than 25 μM.