Title:ATRA Entrapped in DSPC Liposome Enhances Anti-metastasis Effect on Lung and Liver During B16F10 Cell Line Metastasis in C57BL6 Mice
VOLUME: 17 ISSUE: 6
Author(s):Reshma Mahima Reji, Siddikuzzaman and Berlin Grace V.M*
Affiliation:Department of Biotechnology, Karunya University, Coimbatore-641 114, Tamil Nadu, Department of Biotechnology, Karunya University, Coimbatore-641 114, Tamil Nadu, Department of Biotechnology, Karunya University, Karunya Nagar, Coimbatore - 641 114. Tamil Nadu
Keywords:All trans retinoic acid (ATRA), DSPC, hexosamine, liposome, metastasis, sialic acid.
Abstract:Background: The high mortality rate of lung cancer is highly associated with faster metastasis spread.
All Trans Retinoic Acid (ATRA), being the first choice drug for leukemia therapy is now under intense study for
its therapeutic efficiency in other solid cancers.
Objectives: This study was aimed to investigate the anti-metastasis activity of free ATRA and liposome
entrapped ATRA (5:4:1) in the experimental C57BL/6 mice model developed by the injection of B16F10 cell
line into the tail vein.
Method: The ATRA drug was given via i.p for 21 days. The visual lung and liver metastatic tumor nodules were
noted. Various biochemical markers of cancer metastasis in the serum as well as tissues were also analyzed after
sacrifice.
Results: Tumor nodules have significantly decreased in ATRA treatment groups (32.83 ± 1.83 for free ATRA,
23 ± 2.36 for DSPC Lipo-ATRA) when compared with metastasis control (63.16 ± 2.9) in the lungs. Among the
treatment groups, the DSPC lipo-ATRA treated group showed a significant tumor growth inhibition (63.6%) than
that of in the free ATRA treated groups (48%). Similar anti-metastatic effect was observed in liver also. Furthermore
lipo-ATRA has shown a significant change in the levels of biochemical cancer markers analyzed in this study.
Conclusion: Our results concluded that the liposome encapsulated ATRA has an enhanced anti-metastasis
potency than the free ATRA during B16F10 metastatic cell line implantation.
