Title:Synthetic Mono/di-halogenated Coumarin Derivatives and Their Anticancer Properties
VOLUME: 17 ISSUE: 2
Author(s):Kabange Kasumbwe, Katharigatta N. Venugopala, Viresh Mohanlall and Bharti Odhav
Affiliation:Department of Biotechnology and Food Technology, Durban University of Technology, Steve Biko Campus, Durban 4001
Keywords:Synthetic coumarins, MTT assay, anticancer, apoptosis.
Abstract:Background: Coumarins are naturally occurring plant metabolites and several synthetic coumarin analogues
are known for their various pharmacological properties such as anticoagulant, antimicrobial, anticancer, antioxidant,
anti-inflammatory and antiviral properties.
Objective; Keeping this promising pharmacological properties in mind, in the present investigation, mono/dihalogenated
coumarin analogues CMRN1-CMRN7 have been synthesized and evaluated for their anticancer activity.
Method: The cytotoxicity potential of the test compounds was evaluated against UACC-62, MCF-7 and PBM (Peripheral
Blood Mononuclear) cell lines using MTT assay. The apoptotic potential of the coumarin compounds was evaluated
against UACC-62 cell by assessing membrane change, mitochondria membrane potential, pro-apoptotic changes were
investigated using the AnnexinV-PI staining, JC-1, caspase-3 enzyme kits respectively on flow cytometer.
Results: The test compounds CMRN1, CMRN2, CMRN4 and CMRN5 have strongly suppressed the cell proliferation
of UACC-62 and MCF-7 cancer cell lines. Furthermore the test compounds CMRN1, CMRN2, CMRN4 and CMRN5
exerted antiproliferative effects through apoptosis induction against UACC-62.
Conclusion: Compounds CMRN1, CMRN2, CMRN4 and CMRN5 can be considered as lead compounds to arrive at a
promising anticancer agents.
