Background: Carbamate compounds have attracted a great deal of interest in medicinal
chemistry due to their inhibition potential against cholinesterase enzymes.
Method: Hence, this study was undertaken to synthesize new piperazine derivatives including
dithiocarbamate moiety, which is the bioisoster of carbamate. Twenty eight 4-(dimethylaminoalkyl)
piperazine-1-carbodithioate derivatives (3a-3n, 4a-4n) were synthesized. Chemical structures of
these compounds were confirmed by spectral data. Ellman’s assay was applied in order to investigate
inhibitory potency of the compounds against Acetylcholinesterase (AChE) and Butyrylcholinesterase
Results and Conclusion: It was determined that some of the compounds have remarkable activity on
AChE. ADME (Absorption, distribution, metabolism, elimination) predictions were theoretically
performed for all compounds in the series. Enzyme kinetics and molecular docking studies were carried
out for the most active compound (3n) and nature of inhibition and interactions between enzyme
and ligand were described.