Development and Validation of a New HPLC Method For the Detection of 5- Fluorouracil in Mobile Phase and in Plasma

Author(s): Muhammad Hanif*, Muhammad Faheem Akhtar*, Shabana Naeem, Muqeet Wahid, Muhammad Ahmad Shehzad, Malik Saadullah, Bushra Nasir, Samina Afzal

Journal Name: Current Pharmaceutical Analysis

Volume 14 , Issue 1 , 2018

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Graphical Abstract:


Objective: To establish a particular, sensitive and accurate High Performance Liquid Chromatography (HPLC) technique for the specific and effective analysis of 5-Fluorouracil (5-FU) in mobile phase and in plasma.

Method: Analytical methods for 5-FU in mobile phase and plasma were developed. An assay consisted of isocratic elution of 5-FU in prepacked column discovery R-HS C-18, 5micrometer, Catalog # 568523-U, column dimensions : 25cm x 4.6mm using mobile phase composed of 5 mmol/L potassium dihydrogen phosphate (pH 6.0) and methanol at a ratio of 96:4 v/v respectively. 5-FU was detected at 254 nm with the flow rate of 1 ml/min in both mobile phase and plasma.

Results: Retention time was found to be 3.457 minutes in mobile phase and 2.543 minutes in plasma. System suitability test showed no robustness among the inter-day, intra-day analysis, mobile phase and column. Accuracy of the developed method proved reliability of the purposed procedure. %RSD analysis was found to be less than 2% and coefficient of correlation was found to be 1.

Conclusion: Valuable precision, recovery and accuracy were observed indicating method reliability. Method can also be applicable for bioequivalence study.

Keywords: 5-Fluorouracil, HPLC, mobile phase, plasma, linearity, method validation.

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Article Details

Year: 2018
Published on: 11 December, 2017
Page: [3 - 7]
Pages: 5
DOI: 10.2174/1573412912666160922124552
Price: $65

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