Introduction: The antioxidant activity of two potential antioxidants N-ferrocenylmethyl-2-
nitroaniline (2FMNA) and N-ferrocenylmethyl-3-nitroaniline (3FMNA) was evaluated using spectrophotometrical
and electrochemical technics.
Methods: Spectrophotometrical assays were carried out using stable 1,1-diphenyl-2-picrylhydrazyl radicals
(DPPH) and electrochemical assays were based on measuring the oxidation peak current of relatively
stable superoxide anion radical O2
˙¯ electrochemically generated by reduction of commercial molecular
oxygen in DMF.
Results: 2FMNA shows highest antioxidant activity in both methods DPPH and O2
radicals scavenging activities (4.27 ± 0.01mM) and (9.38± 0.09 mM) respectively which is almost equal to that of standard
antioxidant α-tocopherol (4.81± 0.03 mM) and (9.50± 0.09 mM). The diffusion coefficients of the free
˙¯ bound forms of 2FMNA and 3FMNA were evaluated from Randles–Sevcik equation. Cyclic
voltammetry based assay was developed for the calculation of binding parameters like binding constant,
ratio of binding constants and binding free energy.