Background: A new, simple, specific and sensitive reverse-phase liquid chromatographic
method was developed and validated for the estimation of felodipine (FDP) in rabbit plasma using fluorescence
Materials and Methods: Plasma standard were prepared using a simple and one-step protein precipitation
method and 50 μ l of the standard solutions were injected into the column. The method was found to
be linear in the concentration range of 10–1000 ng/ml. The developed bioanalytical method was validated
as per standard guidelines. Validation study results demonstrated accuracy, precision, sensitivity
and selectivity of the proposed method.
Results: The developed method was successfully employed in the determination of FDP in plasma samples
obtained from the pharmacokinetic study of the immediate release tablets in rabbits.
Keywords: Felodipine, fluorescence detector, high-performance liquid chromatography, rabbit plasma, pharmacokinetic study,
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