Determination of Optimum Separation Condition for Some Carbapenem Antibiotics Using RPLC with the Aid of Central Composite Design and Desirability Function

Author(s): Busra Gundogan, Ebru C. Demiralay, Yasar D. Daldal, Zehra Ustun

Journal Name: Current Pharmaceutical Analysis

Volume 13 , Issue 2 , 2017

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Graphical Abstract:


Background: In order to acquire the best chromatographic condition with maximum resolution and minimum analysis time, the central composite design (CCD) added with Derringer’s desirability function was utilised in order to separation of doripenem, meropenem and ertapenem.

Experiment: The parameters (mobile phase pH values, column temperature and concentration of acetonitrile) affecting separation of these compounds were selected. The first order effects for each factor and their interactions were investigated. Enhanced method was applied correctly for concurrent determination of doripenem, meropenem and ertapenem in human urine, using a X Terra C18 (5 µm, 250 mm x 4,6 mm ID) column. In accordance with ICH guidelines to verify linearity, accuracy and precision.

Result: By using equations obtained CCD, protonation constant values (pKa) of doripenem, meropenem and ertapenem were also predicted.

Keywords: Carbapenem group, predicted pKa values, central composite design (CCD), Derringer’s desirability, optimization, RPLC, method validation.

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Article Details

Year: 2017
Published on: 17 January, 2017
Page: [100 - 109]
Pages: 10
DOI: 10.2174/1573412912666160822114927
Price: $65

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