Background: In order to acquire the best chromatographic condition with maximum resolution
and minimum analysis time, the central composite design (CCD) added with Derringer’s desirability
function was utilised in order to separation of doripenem, meropenem and ertapenem.
Experiment: The parameters (mobile phase pH values, column temperature and concentration of acetonitrile)
affecting separation of these compounds were selected. The first order effects for each factor and
their interactions were investigated. Enhanced method was applied correctly for concurrent determination
of doripenem, meropenem and ertapenem in human urine, using a X Terra C18
(5 µm, 250 mm x 4,6 mm
ID) column. In accordance with ICH guidelines to verify linearity, accuracy and precision.
Result: By using equations obtained CCD, protonation constant values (pKa
) of doripenem, meropenem
and ertapenem were also predicted.