Title:Antiproliferative and Apoptotic Effect of Dendrosomal Curcumin Nanoformulation in P53 Mutant and Wide-Type Cancer Cell Lines
VOLUME: 17 ISSUE: 5
Author(s):Maryam Montazeri, Younes Pilehvar-Soltanahmadi, Mina Mohaghegh, Alireza Panahi, Samaneh Khodi, Nosratollah Zarghami* and Majid Sadeghizadeh*
Affiliation:Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Department of Molecular Biology and Biotechnology, University of Aix-Marseille, Marseille, Department of Biology Faculty of Basic Sciences University of Mohaghegh Ardabili, Ardabil, Department of Medical Genetic, National Institute of Genetic Engineering and Biotechnology, Tehran, Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran
Keywords:Dendrosomal curcumin (DNC), p53 mutant, apoptosis, breast cancer, colon cancer.
Abstract:Objective: The aim of this paper is to investigate the effect of dendrosomal curcumin (DNC) on the
expression of p53 in both p53 mutant cell lines SKBR3/SW480 and p53 wild-type MCF7/HCT116 in both RNA and
protein levels.
Background: Curcumin, derived from Curcumin longa, is recently considered in cancer related researches for its cell
growth inhibition properties. p53 is a common tumor-suppressor gene involved in cancers and its mutation not only
inhibits tumor suppressor activity but also promotes oncogenic activity.
Method: Here, p53 mutant/Wild-type cells were employed to study the toxicity of DNC using MTT assay, Flow
cytometry and Annexin-V, Real-time PCR and Western blot were used to analyze p53, BAX, Bcl-2, p21 and Noxa
changes after treatment.
Results: During the time, DNC increased the SubG1 cells and decreased G1, S and G2/M cells, early apoptosis also
indicated the inhibition of cell growth in early phase. Real-Time PCR assay showed an increased mRNA of BAX,
Noxa and p21 during the time with decreased Bcl-2. The expression of p53 mutant decreased in SKBR3/SW480, and
the expression of p53 wild-type increased in MCF7/HCT116.
Conclusion: Consequently, p53 plays an important role in mediating the survival by DNC, which can prevent tumor
cell growth by modulating the expression of genes involved in apoptosis and proliferation.
