An Antileukemic Glutaminase Free L-Asparaginase from Bacillus brevis

Author(s): Wamik Azmi, Umesh Kumar Narta, Nishant Singh, Vibhor Gupta, Shamsher Singh Kanwar, Sarita Devi

Journal Name: Current Biotechnology

Volume 6 , Issue 1 , 2017

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Abstract:

Background: The antitumor activity of L-asparaginase isolated from natural sourceshas been proven thriving in treatment of acute lymphoblastic leukemia and other lymphoproliferative disorders however production efficiency and associated glutaminase activity has always been a limitation to its use. Asparaginase also induces apoptosis and cytoprotective autophagy in chronic myeloid leukemia cells. Together these findings make L-asparaginase a useful drug for the treatment of Acute Lymphocytic Leukemia and various other tumors.

Methods: In the present study, the purification and characterization of a glutaminasefree intra-cellular L-asparaginase was done from Bacillus brevis, originally isolated from a cold desert region of Himachal Pradesh, India.

Results: The enzyme was purified to 90-fold by a SulphopropylSephadex columnchromatography and found to be 32 kDa in molecular weight under reduced conditions. The enzyme possessed significant amount of activity at human physiological conditions of temperature (37°C) and pH (7.4) and have a half-life of 70 minutes at 25ºC. The Km and Vmax values of L-asparaginase were found to be 3.5 X 10-2 M and 0.77 IU, respectively. The L-asparaginase purified from B. brevis showed comparable in vitro toxicity on Hep-2C cell lines (91% survival) in comparison to commercial L-asparaginase preparation (90% survival) obtained from E. coli.

Conclusion: L-asparaginase with high purity and high affinity for L-asparagine will bemost suitable for the treatment of lymphoblastic leukemia. The glutaminase free L-asparaginase obtained from B. brevis thus provides an additional therapeutic agent and could be used against for the treatment of various malignant diseases.

Keywords: Acute Lymphoblastic Leukemia (ALL). Bacillus brevis, glutaminase free L-asparaginase, intracellular enzyme, one-step purification, MTT assay.

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Article Details

VOLUME: 6
ISSUE: 1
Year: 2017
Page: [58 - 68]
Pages: 11
DOI: 10.2174/2211550105666160805102153
Price: $65

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