The canine metabolic diseases, such as obesity and diabetes, have become
a worldwide problem. Fibroblast growth factor 21 (FGF21) is a potent regulator
which has many biological functions relative to metabolism regulation. It
suggests that FGF21 plays important roles in regulating canine metabolic diseases.
To acquire the recombinant canine FGF21 (rcFGF21) in Escherichia coli, the
recombinant bacteria were induced by 0.5 mM IPTG for 16 hours at 16 °C, and
the rcFGF21 protein was purified by Ni-NTA. 8 mg rcFGF21 was acquired from
one liter bacteria. The rcFGF21 protein has specific immunoblot reactivity against
anti-FGF21 and anti-His antibody. The in vivo experimental result showed that
rcFGF21 can significantly reduce plasma glucose of STZ-induced diabetic mice.
Keywords: Recombinant canine FGF21, Escherichia coli, purification, plasma glucose.
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