Background: Fisetin,quercetin and kaempferol are among the important representatives of flavonols,
biological active phytocomounds, with low water solubility.
Objective: To evaluate the antimicrobial effect, respectively the antiproliferative and pro apoptotic activity on the
B164A5 murine melanoma cell line of pure flavonols and their beta cyclodextrins complexes.
Method: Incorporation of fisetin, quercetin and kaempferol in beta cyclodextrins was proved by scanning electron
microscopy (SEM), differencial scanning calorimetry (DSC) and X-ray powder diffraction (XRPD). Pure compounds
and their complexes were tested for antiproliferative (MTT) and pro-apoptotic activity (Annexin V-PI) on the B164A5
murine melanoma cell line and for the antimicrobial properties (Disk Diffusion Method) on the selected strains.
Results: The phytocompounds presented in a different manner in vitro chemopreventive activity against B164A5
murine melanoma cell line and weak antimicrobial effect.
Conclusion: The three flavonols: fisetin, quercetin and kaempferol were successfully incorporated in beta-cyclodextrin
(BCD) and hydroxylpropyl-beta-cyclodextrin (HPBCD). Incorporation in beta cyclodextrins had a mix effect on the
biological activity conducing to decrease, increase or consistent effect compared to pure phytocompound, depending
on the screened process and on the chosen combination.