Re-SILAC: A Simple and Convenient Approach for the Preparation of the SILAC Internal Standards

Author(s): Tianying Yu, Tailong Yi, Miaomiao Tian, Zhongwei Xu, Yao Zhang, Linhui Zhai, Xiaoming Yang, Ping Xu

Journal Name: Current Proteomics

Volume 13 , Issue 3 , 2016


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Abstract:

SILAC (Stable Isotope Labeling with Amino acids in Cell culture) is one of the most popular methods in quantitative proteomics field. The internal standards of SILAC can mark a variety of clinical samples or tissues instead of labeling the whole organism and play an important role in proteomics. Preparing the SILAC internal standards has been difficult and time-consuming for culturing cell lines, therefore a simple and convenient Re-SILAC method was introduced. Cell lines labeled with SILAC were thawed in SILAC medium to be remarked again. Comparing the morphology, size, growth curve and proteome of MHCC97-L between Re-SILAC and SILAC cell lines, it was found that SILAC-labeled cells that were frozen with liquid nitrogen had no influence on Re- SILAC-labeled. In conclusion, Re-SILAC method is a useful method for preparing SILAC internal standards.

Keywords: CCK-8, liquid storage, MHCC97-L, quantitative proteomics, Re-SILAC, SILAC.

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Article Details

VOLUME: 13
ISSUE: 3
Year: 2016
Published on: 09 June, 2016
Page: [237 - 240]
Pages: 4
DOI: 10.2174/1570164613666160609130437
Price: $25

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