Title:Identification and Characterization of Follistatin-Related Protein-1 Involved in the Regulation of Chinese Perch Skeletal Muscle Hyperplasia
VOLUME: 16 ISSUE: 6
Author(s):W. Chu, J. Cheng, X. Zhu, P. Wu, L. Chen, J. Wang, Y. Wu, M. Zeng and J. Zhang
Affiliation:Department of Bioengineering and Environmental Science, Changsha University, Changsha, Hunan 410003, China.
Keywords:Siniperca chuatsi, follistatin-related protein, myostatin, satellite cell, muscle growth, hyperplasia.
Abstract:Follistatin-related protein (FSRP), which belongs to a member of
the follistatin family, has been postulated to be a new negative regulator of
myostatin (MSTN) and is involved in muscle development. In this study, we
cloned the complete cDNA sequence of FSRP-1 from Chinese perch
muscle. FSRP-1 mRNA was highly expressed in the fast muscle and brain
tissue of adult fish. The expression of FSRP-1 rapidly increased at 90 days
post hatching (dph) in the fast muscle of Chinese perch. Furthermore, to
investigate the roles of FSRP-1 in muscle growth, we constructed a FSRP-
1 expression vector and isolated FSRP-1 fusion protein. The purified
FSRP-1 fusion protein was injected into the muscle tissues of the Chinese
perch (90 dph). The results showed that the number of muscle fibers and the satellite cell
activation frequency were increased in the FSRP-1 treatment group. In addition, the
myostatin (MSTN) expression was significantly decreased upon the FSRP-1 treatment.
Collectively, the results suggest a possible mechanism of the FSRP-1 for inhibiting MSTN
activity and enhancing muscle growth and renewal in vivo, and it may provide an applicable
implication for the defected muscle repairing and regeneration.
