Background: Previous studies have shown that catabolism of adenosine
5’-triphosphate (ATP) in red blood cell (RBC) may be a key factor for cardiovascular
protection and maintaining cardiovascular homeostasis.
Objective: To investigate the effect of cardiovascular injury on adenosine and ATP
catabolism in systemic blood using a freely moving rat model in vivo.
Method: After acclimatized to the experimental environment, Sprague Dawley (SD)
rats were each given either isoproterenol (30 mg/kg) or saline (1 mL/kg) by subcutaneous
(sc) injection. Blood samples were collected sequentially for up to 6 hours
for measurement of red blood cell (RBC) concentrations of adenine nucleotides and
plasma concentrations of adenosine and its oxypurine metabolites.
Results: We have found isoproterenol induced 50% mortality under the experimental condition. Plasma
concentrations of adenosine (ADO) and uric acid (UA) and red blood cell (RBC) concentrations of
adenosine 5’-diphosphate (ADP) and adenosine 5’-monophosphate (AMP) in RBC were significantly
higher in the isoproterenol treated rats (p < 0.05 for all the comparison). On the other hand, plasma concentrations
of hypoxanthine (HYP) were higher in the control group (p < 0.05), but there was no statistically
significant changes in ATP concentrations in the RBC (p > 0.05).
Conclusion: Cardiovascular injury induced by isoproterenol resulted in breakdown of ATP to ADP and
AMP in the RBC and also breakdown of ADO to UA in plasma and other tissues.