The immune response of the host tissue to the tested biomaterial predicts
the success of implantation therapy success. Connective tissue cell line SaOS-2 and
mononuclear cells isolated from “buffy coat” testing determines the overall immune
response to the implanted biomaterial. Alginate hydrogel coatings doped with inorganic
hydroxyapatite (Ti/Alg/HAP) or beta-tricalcium phosphate (Ti/Alg/TCP)
nanoparticles on Titanium grade 2 were prepared. Both cell types mentioned above
were used to evaluate the cytokine production when cultivated on the proposed coatings.
The pristine Ti and alginate (Alg) served as control surfaces. Cytokine production
was assessed by the multiplex proteomic analysis of 40 cytokines.
Mononuclear cell testing revealed differences between control (Ti, Ti/Alg) and alginate
hydrogel surfaces doped with calcium phosphates (Ti/Alg/TCP and Ti/Alg/HAP). Cytokine production
declines from the highest found on the mononuclear cells treated by Ti/Alg/HAP through control Ti
and Ti/Alg/TCP surface to control Ti/Alg surface with lowest cytokine production. Mononuclear cells
produced mostly IL-6 and IL-8.
No significant differences between Ti/Alg/TCP and Ti/Alg/HAP were found in cytokines produced by
cell line SaOS-2. Cell line SaOS-2 produced a wide spectrum of cytokines, but the production was low,
with the exception of TIMP-2.
The comparison of cytokines produced by cell line SaOS-2 and mononuclear cells from “buffy coat”
indicated that mononuclear cells have much better potential to show differences between the monitored
materials. The immune response of mononuclear cells showed differences between tested materials,
whereas SaOS-2 cells were not sufficiently sensitive. Therefore, besides SaOS-2 cells, mononuclear cells
should also be considered for in vitro evaluation of overall immune response induced by the presence of