Aim: The aim of the present study is to prepare Diltiazem HCl loaded Bovine Serum
Albumin nanoparticles by Desolvation technique using Isopropanol as desolvating agent.
Here in this study, attempts have been made to prepare Diltiazem loaded BSA nanoparticles
by Desolvation technique.
Methodology: Two methods were followed in the process of adding desolvating agent to
aqueous solution of BSA; continuous addition method and intermittent addition method. In
continuous addition method, the desolvating agent was added at the rate of 1ml per minute,
whereas, in intermittent addition method, the desolvating agent was added at the rate of 1ml
per every five minutes. The effects of continuous and intermittent addition methods on
nanoparticles size, stability, loading capacity, encapsulation efficiency and in vitro drug release
were studied. The particle size and stability of the formulations were determined by Horiba
SZ 100 series particle size analyzer. Encapsulation efficiency and loading capacity were
determined by ELTEK NP 400 Ultracentrifuge.
Results: The particle size was 267.3nm and 257.8nm, and encapsulation efficiency was found
to be 90.3% and 95% Comparative study was performed to determine the best method for the
preparation of Diltiazem nanoparticles.
Conclusion: On comparison, intermittent addition method was considered to be the best
method for the preparation of Diltiazem HCl loaded BSA nanoparticles because of the
smaller particle size and greater stability and encapsulation efficiency. The drug release was
sustained till 12hrs for nanoparticles prepared by intermittent addition method.