Identification and Quantitation of Some Characteristic Phenolic Compounds in Elderberry Juice by HPLC with Coulometric Electrode Array Detection

Author(s): Norbert Neger, Heidi E. Schwartz-Zimmermann, Gerhard Sontag

Journal Name: Current Bioactive Compounds

Volume 12 , Issue 4 , 2016

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Abstract:

An HPLC-coulometric electrode array detection (CEAD) based method for the determination of protocatechuic acid, chlorogenic acid, rutin (quercetin-3-O-rutinoside), nicotiflorin (kaempferol-3-Orutinoside) and quercetin in elderberry juice was developed. Sample work up for the determination of phenolic acids included solid phase extraction (SPE). For the analysis of flavonoids, simple dilution and centrifugation steps were sufficient. The compounds were separated isocratically on the same reversed phase column using two different mobile phases to prevent long retention times and tailing of the aglycone peaks. The analytes were then detected at twelve coulometric cells. The current/voltage curves contributed to compound identification and to verification of the peak purity of the separated compounds. Additionally, LC-ESI-MS in the negative ion scan mode was applied for qualitative analysis. Quantification of the compounds was carried out by standard addition method. The content in elderberry juice varied between 785 mg/l for rutin and 2.8 mg/l for quercetin. The presented rapid and reliable method can be used to differentiate between elderberry juices of different origins. Coulometric electrode array detection was applied for the first time for the determination of these compounds in elderberry juice.

Dedicated to em. O. Univ. Prof. Dr. Adolf Neckel on the occasion of his 90th birthday.

Keywords: Phenolic compounds, identification, quantitation, HPLC-CEAD, HPLC-ESI-MS, elderberry juice.

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Article Details

VOLUME: 12
ISSUE: 4
Year: 2016
Page: [251 - 257]
Pages: 7
DOI: 10.2174/1573407212666160512115921
Price: $65

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