Abstract
Isotope dilution mass spectrometry (IDMS) is based on a simple and straightforward idea of using an isotope labeled compound as an internal standard for quantitation purposes. IDMS now competes with routine quantitative methods such as immunoassays (ELISA, RIA) due to its high precision, accuracy and robustness. The isotope dilution concept allows for protein quantitation and has been showing great promise in clinical application in the last decade. The present review contains a short description of the current status of proteomics and principles of absolute quantitative methodologies; it focuses on some mass spectrometric techniques, sample preparation and recent examples of IDMS applications for peptide and protein quantitation in clinical chemistry.
Keywords: Isotope dilution analysis, multi-dimensional separations, proteomics.
Current Proteomics
Title:Quantitative Proteomics with Isotope Dilution Analysis: Principles and Applications
Volume: 13 Issue: 1
Author(s): Kuanysh Kabytaev and Alexander Stoyanov
Affiliation:
Keywords: Isotope dilution analysis, multi-dimensional separations, proteomics.
Abstract: Isotope dilution mass spectrometry (IDMS) is based on a simple and straightforward idea of using an isotope labeled compound as an internal standard for quantitation purposes. IDMS now competes with routine quantitative methods such as immunoassays (ELISA, RIA) due to its high precision, accuracy and robustness. The isotope dilution concept allows for protein quantitation and has been showing great promise in clinical application in the last decade. The present review contains a short description of the current status of proteomics and principles of absolute quantitative methodologies; it focuses on some mass spectrometric techniques, sample preparation and recent examples of IDMS applications for peptide and protein quantitation in clinical chemistry.
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Cite this article as:
Kabytaev Kuanysh and Stoyanov Alexander, Quantitative Proteomics with Isotope Dilution Analysis: Principles and Applications, Current Proteomics 2016; 13 (1) . https://dx.doi.org/10.2174/1570164613666160413121219
DOI https://dx.doi.org/10.2174/1570164613666160413121219 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
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