An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding

Author(s): Paul Rees, M. Rowan Brown, John W. Wills, Huw Summers

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 19 , Issue 5 , 2016

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Many applications in biomedical research require the long-term identification and tracking of cells over time. In previous work we have demonstrated that by sequentially dosing a cell population with different emission wavelength nanoparticles it is possible to use the random number of nanoparticle loaded vesicles generated by the cells as a barcode for individual cells within the population. In this paper we develop a simple model to describe the number of codes that can be generated using this sequential loading protocol. The methodology is validated by comparison with experiment and subsequently used to predict the effect of varying the number of colors used to encode the cells and also to assess the effect of misreading the cellular code due to errors in imaging the vesicles.

Keywords: Microscopy, nanoparticles, time-lapse, barcoding.

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Article Details

Year: 2016
Published on: 10 May, 2016
Page: [362 - 369]
Pages: 8
DOI: 10.2174/1386207319666160408150649
Price: $65

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