Exploring Polypharmacology in Drug Discovery and Repurposing Using the CANDO Platform

Author(s): Gaurav Chopra, Ram Samudrala

Journal Name: Current Pharmaceutical Design

Volume 22 , Issue 21 , 2016

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Background: Traditional drug discovery approaches focus on a limited set of target molecules for treatment against specific indications/diseases. However, drug absorption, dispersion, metabolism, and excretion (ADME) involve interactions with multiple protein systems. Drugs approved for particular indication(s) may be repurposed as novel therapeutics for others. The severely declining rate of discovery and increasing costs of new drugs illustrate the limitations of the traditional reductionist paradigm in drug discovery. Methods: We developed the Computational Analysis of Novel Drug Opportunities (CANDO) platform based on a hypothesis that drugs function by interacting with multiple protein targets to create a molecular interaction signature that can be exploited for therapeutic repurposing and discovery. We compiled a library of compounds that are human ingestible with minimal side effects, followed by an ‘all-compounds’ vs ‘all-proteins’ fragment-based multitarget docking with dynamics screen to construct compound-proteome interaction matrices that were then analyzed to determine similarity of drug behavior. The proteomic signature similarity of drugs is then ranked to make putative drug predictions for all indications in a shotgun manner. Results: We have previously applied this platform with success in both retrospective benchmarking and prospective validation, and to understand the effect of druggable protein classes on repurposing accuracy. Here we use the CANDO platform to analyze and determine the contribution of multitargeting (polypharmacology) to drug repurposing benchmarking accuracy. Taken together with the previous work, our results indicate that a large number of protein structures with diverse fold space and a specific polypharmacological interactome is necessary for accurate drug predictions using our proteomic and evolutionary drug discovery and repurposing platform. Conclusion: These results have implications for future drug development and repurposing in the context of polypharmacology.

Keywords: Shotgun drug discovery, virtual screening, computational proteomics, polypharmacology, multitarget.

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Article Details

Year: 2016
Published on: 25 March, 2016
Page: [3109 - 3123]
Pages: 15
DOI: 10.2174/1381612822666160325121943
Price: $65

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